Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein抗体[RSV5A6] (ab94968)

概述

  • 产品名称Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein抗体[RSV5A6]
  • 描述
    小鼠单克隆抗体[RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein
  • 经测试应用适用于: ELISA, WB, ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Other
  • 免疫原

    Human Respiratory Syncytial Virus strain A2 infected HeLa cells

  • 常规说明Fusion partner: PS-NS/1-Ag4


    ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B.

性能

应用

Our Abpromise guarantee covers the use of ab94968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with Acetone.

靶标

  • 相关性Respiratory Syncytial Virus (RSV) Fusion (F) Glycoprotein is a Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.
  • 细胞定位Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass membrane protein.
  • 别名
    • F antibody
    • Fusion glycoprotein F0 antibody
    • Protein F antibody
    • RSV Fusion (F) Glycoprotein antibody

Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein antibody [RSV5A6] (ab94968)参考文献

This product has been referenced in:
  • West WH  et al. Biological activity, binding site and affinity of monoclonal antibodies to the fusion protein of respiratory syncytial virus. J Gen Virol 75 ( Pt 10):2813-9 (1994). Read more (PubMed: 7931172) »

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