Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein抗体[RSV5A6] (ab94968)
Key features and details
- Mouse monoclonal [RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein
- Suitable for: ELISA, WB, ICC/IF, IHC-P, IHC-Fr
- Reacts with: Respiratory syncytial virus
- Isotype: IgG2a
概述
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产品名称
Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein抗体[RSV5A6]
参阅全部 Respiratory Syncytial Virus Fusion (F) Glycoprotein 一抗 -
描述
小鼠单克隆抗体[RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein -
宿主
Mouse -
经测试应用
适用于: ELISA, WB, ICC/IF, IHC-P, IHC-Frmore details -
种属反应性
与反应: Respiratory syncytial virus -
免疫原
Tissue, cells or virus corresponding to Respiratory Syncytial Virus Fusion (F) Glycoprotein. hRSV strain A2 infected HeLa cells
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常规说明
Fusion partner: PS-NS/1-Ag4ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B. -
克隆
单克隆 -
克隆编号
RSV5A6 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94968于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
Fix with Acetone. |
说明 |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. Fix with Acetone. |
靶标
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相关性
Respiratory Syncytial Virus (RSV) Fusion (F) Glycoprotein is a Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis. -
细胞定位
Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass membrane protein. -
数据库链接
- SwissProt: P03420 Respiratory syncytial virus
- SwissProt: P12568 Respiratory syncytial virus
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别名
- F antibody
- Fusion glycoprotein F0 antibody
- Protein F antibody
- RSV Fusion (F) Glycoprotein antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab94968 被引用在 7 文献中.
- Niyomdecha N et al. Repurposing of antiparasitic niclosamide to inhibit respiratory syncytial virus (RSV) replication. Virus Res 295:198277 (2021). PubMed: 33476693
- Tang W et al. Mechanism of Cross-Resistance to Fusion Inhibitors Conferred by the K394R Mutation in Respiratory Syncytial Virus Fusion Protein. J Virol 95:e0120521 (2021). PubMed: 34379500
- Touzelet O et al. The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins. Mol Cell Proteomics 19:793-807 (2020). PubMed: 32075873
- Li J et al. Long Noncoding RNA NRAV Promotes Respiratory Syncytial Virus Replication by Targeting the MicroRNA miR-509-3p/Rab5c Axis To Regulate Vesicle Transportation. J Virol 94:N/A (2020). PubMed: 32102886
- Li M et al. Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. J Virol 92:N/A (2018). PubMed: 29386287
- Vanover D et al. RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane. Nat Commun 8:667 (2017). PubMed: 28939853
- West WH et al. Biological activity, binding site and affinity of monoclonal antibodies to the fusion protein of respiratory syncytial virus. J Gen Virol 75 ( Pt 10):2813-9 (1994). PubMed: 7931172