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Choosing the optimal affinity resin is key to affinity purification of antibodies, immunoprecipitation and ChIP. Commonly used immobilized proteins vary in their ability to bind antibodies of different species and subclass. Read our detailed selection guide.
Discover our In-Cell ELISA (ICE) detailed protocol. Measure protein levels or post-translational modifications in cultured adherent cells.
Use our blue native electrophoresis protocol to determine the size, relative abundance and subunit composition of mitochondrial protein complexes.
Found in all eukaryotic cells, actin is a protein essential to maintain cell structure. In humans, three main actin groups have been identified - alpha, beta. Actin is used as a loading control for western blotting to normalize protein loading. Read full article.
This guide outlines the steps to perform lauryl-maltoside extractions of functional enzymes and proteins from tissues and cultured cells for use with our ELISA and dipstick activity assays.
Find a complete list of human analytes for our membrane antibody arrays. Use membrane arrays to profile samples for cytokines, angiogenesis markers, inflammatory markers and many more.
Review our range of assays for your plate reader, flow cytometer and microscope.
Read our glossary of definitions for antibody related terms.
Detect multiple targets on the same blot at the same time.
Find out the procedure to block your antibody using an immunizing peptide.