兔多克隆抗体to Repulsive Guidance Molecule B
Recombinant fragment, corresponding to a region within the C terminal amino acids 219-467 of Human Repulsive Guidance Molecule B (NP_001012779)
293T, HeLaS3, HepG2, Molt-4, Raji cell lysates. A431, H1299 whole cell lysates. U87 xenograft.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
1/100 - 1/500.
Member of the repulsive guidance molecule (RGM) family that contributes to the patterning of the developing nervous system (By similarity). Acts as a bone morphogenetic protein (BMP) coreceptor that potentiates BMP signaling (By similarity). Promotes neuronal adhesion (By similarity). May inhibit neurite outgrowth.
Belongs to the repulsive guidance molecule (RGM) family.
Cell membrane. Membrane raft.
Information by UniProt
DRG11-responsive axonal guidance and outgrowth of neurite antibody
Repulsive Guidance Molecule B antibody
Western blot - Repulsive Guidance Molecule B antibody (ab96727)
All lanes : Anti-Repulsive Guidance Molecule B antibody (ab96727) at 1/1000 dilution Lane 1 : A431 whole cell lysate Lane 2 : H1299 whole cell lysate Lane 3 : HeLa S3 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 52 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Repulsive Guidance Molecule B antibody (ab96727)
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ab96727 at 1/100 dilution.
has not yet been referenced specifically in any publications.
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