The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Use at an assay dependent concentration. PubMed: 21467223
Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
Belongs to the repulsive guidance molecule (RGM) family.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Repulsive Guidance Molecule A antibody (ab26287)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
Immunohistochemistical (Formalin/PFA-fixed paraffin-embedded sections) detection of Repulsive Guidance Molecule A antibody (ab26287) in mouse brain. Fixative used: Formaldehyde; Antigen retrieval step: None; Permeabilization: Yes; Blocking step: 1% Serum for 1 hour @ 25°C. Primary Antibody used at 1/500 incubated for 24 h @ 4°C in 0.1% PBS+ 0.3 Triton X+ 1% goat serum. This image shows RGM-A expressed in all six layers of cortical neurons of adult mouse brain.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Repulsive Guidance Molecule A antibody (ab26287)This image is courtesy of an abreview submitted by Sophie Pezet, ESPCI, France
IHC-FoFr image of Repulsive Guidance Molecule A staining in rat cortex (left) and hippocampus (right) using ab26287. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight.
IHC image of Repulsive Guidance Molecule A staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab26287, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Xu X et al. Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS. Hum Vaccin Immunother12:2169-2180 (2016).
Read more (PubMed: 26986456) »
Mirakaj V et al. Repulsive guidance molecule-A (RGM-A) inhibits leukocyte migration and mitigates inflammation. Proc Natl Acad Sci U S A108:6555-60 (2011).
Read more (PubMed: 21467223) »