RabMAb

Anti-RENT1/hUPF1抗体[EPR4681] (ab109363)

概述

  • 产品名称Anti-RENT1/hUPF1抗体[EPR4681]
    参阅全部 RENT1/hUPF1 一抗
  • 描述
    兔单克隆抗体[EPR4681] to RENT1/hUPF1
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种��反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in human RENT1/hUPF1.

  • 阳性对照
    • HuT-78, Raji, SH-SY5Y and HeLa cell lysates, Human kidney tissue, Human transitional cell carcinoma tissue, HeLa cells.
  • 常规说明

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR4681
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 124 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250. 

靶标

  • 功能Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • 结构域The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • 翻译后修饰Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent helicase RENT1 antibody
    • ATP-dependent helicase RENT1 antibody
    • Delta helicase antibody
    • FLJ43809 antibody
    • FLJ46894 antibody
    • HUPF 1 antibody
    • hUpf1 antibody
    • KIAA0221 antibody
    • Nonsense mRNA reducing factor 1 antibody
    • NORF 1 antibody
    • NORF1 antibody
    • pNORF 1 antibody
    • pNORF1 antibody
    • Regulator of nonsense transcripts 1 antibody
    • RENT 1 antibody
    • RENT1 antibody
    • RENT1_HUMAN antibody
    • Smg 2 antibody
    • Smg 2 homolog nonsense mediated mRNA decay factor antibody
    • UP Frameshift 1 antibody
    • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
    • Up frameshift mutation 1 homolog antibody
    • Up frameshift suppressor 1 homolog antibody
    • Up-frameshift suppressor 1 homolog antibody
    • UPF 1 antibody
    • UPF 1 regulator of nonsense transcripts homolog antibody
    • upf1 antibody
    • UPF1 regulator of nonsense transcripts homolog antibody
    • UPF1 RNA helicase and ATPase antibody
    • Yeast Upf1p homolog antibody
    see all

Anti-RENT1/hUPF1 antibody [EPR4681] 图像

  • All lanes : Anti-RENT1/hUPF1 antibody [EPR4681] (ab109363) at 1/10000 dilution

    Lane 1 : HuT-78 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 124 kDa
  • ab109363, at 1/100, staining RENT1/hUPF1 in Human kidney tissue by immunohistochemistry.
  • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling RENT1/hUPF1 with purified ab109363 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control

  • ab109363, at 1/100, staining RENT1/hUPF1 in Human transitional cell carcinoma tissue by immunohistochemistry.
  • ab109363, at 1/100, staining RENT1/hUPF1 in HeLa cells by immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab109363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109363, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-RENT1/hUPF1 antibody [EPR4681] (ab109363)参考文献

This product has been referenced in:
  • Miller JN  et al. The role of nonsense-mediated decay in neuronal ceroid lipofuscinosis. Hum Mol Genet 22:2723-34 (2013). WB ; Human . Read more (PubMed: 23539563) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10% SDS-polyacrylamide gell)
Sample Human Cell lysate - whole cell (Immortalized lymphoblast cell)
Specification Immortalized lymphoblast cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Jake Miller

Verified customer

提交于 Jul 08 2013

Western blot

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (NuPAGE® Novex® 3-8% Tris-Acetate Gel 1.0 mm)
Sample Human Cell lysate - whole cell (U2OS cells)
Specification U2OS cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Melanie Blasius

Verified customer

提交于 May 20 2013

Thank you for contacting us. While we do carry a number of anti-hUPF1 antibodies which have been generated using immunogens near the C-terminus, the epitope for ab109363 is generated from a region near the N-terminus. A number of our other anti-...

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Thank you for contacting Abcam about ab109363, ab92655 and ab10534. Unfortunately the immunogen sequences for these three antibodies is proprietary information and I do not have access to that information. Please except my apologies for n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"