概述

  • 产品名称Anti-RENT1/hUPF1抗体
    参阅全部 RENT1/hUPF1 一抗
  • 描述
    山羊多克隆抗体to RENT1/hUPF1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human regulator of nonsense transcripts 1 (RENT1)(LocusLink ID 5976).

  • 阳性对照
    • Whole cell lysate from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibodies were affinity purified using the peptide immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2500. Detects a band of approximately 135 kDa (predicted molecular weight: 134 kDa).

靶标

  • 功能Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • 结构域The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • 翻译后修饰Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent helicase RENT1 antibody
    • ATP-dependent helicase RENT1 antibody
    • Delta helicase antibody
    • FLJ43809 antibody
    • FLJ46894 antibody
    • HUPF 1 antibody
    • hUpf1 antibody
    • KIAA0221 antibody
    • Nonsense mRNA reducing factor 1 antibody
    • NORF 1 antibody
    • NORF1 antibody
    • pNORF 1 antibody
    • pNORF1 antibody
    • Regulator of nonsense transcripts 1 antibody
    • RENT 1 antibody
    • RENT1 antibody
    • RENT1_HUMAN antibody
    • Smg 2 antibody
    • Smg 2 homolog nonsense mediated mRNA decay factor antibody
    • UP Frameshift 1 antibody
    • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
    • Up frameshift mutation 1 homolog antibody
    • Up frameshift suppressor 1 homolog antibody
    • Up-frameshift suppressor 1 homolog antibody
    • UPF 1 antibody
    • UPF 1 regulator of nonsense transcripts homolog antibody
    • upf1 antibody
    • UPF1 regulator of nonsense transcripts homolog antibody
    • UPF1 RNA helicase and ATPase antibody
    • Yeast Upf1p homolog antibody
    see all

Anti-RENT1/hUPF1 antibody 图像



  • Predicted band size : 134 kDa


    ab10534 Detection of Human RENT1 by Western blot. Samples: Whole cell lysate (30 mg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10534 used at 1 ug/ml. Detection: Chemiluminescence.

Anti-RENT1/hUPF1 antibody (ab10534)参考文献

This product has been referenced in:
  • Zhao C  et al. Stress-sensitive regulation of IFRD1 mRNA decay is mediated by an upstream open reading frame. J Biol Chem 285:8552-62 (2010). WB ; Human . Read more (PubMed: 20080976) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. While we do carry a number of anti-hUPF1 antibodies which have been generated using immunogens near the C-terminus, the epitope for ab109363 is generated from a region near the N-terminus. A number of our other anti-...

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Thank you for contacting Abcam about ab109363, ab92655 and ab10534. Unfortunately the immunogen sequences for these three antibodies is proprietary information and I do not have access to that information. Please except my apologies for n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"