概述

  • 产品名称Anti-Renin抗体
    参阅全部 Renin 一抗
  • 描述
    小鼠单克隆抗体to Renin
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 24-407 of Human Renin

性能

应用

Our Abpromise guarantee covers the use of ab57635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
  • 疾病相关Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
    Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
  • 序列相似性Belongs to the peptidase A1 family.
  • 细胞定位Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Angiotensin forming enzyme antibody
    • Angiotensin forming enzyme precursor antibody
    • Angiotensinogenase antibody
    • Angiotensinogenase precursor antibody
    • FLJ10761 antibody
    • HNFJ2 antibody
    • REN antibody
    • Ren1 antibody
    • RENI_HUMAN antibody
    • Renin antibody
    • Renin precursor renal antibody
    see all

Anti-Renin antibody 图像



  • Predicted band size : 45 kDa
    Renin antibody (ab57635) at 1ug/lane + K-562 cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab57635 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57635, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Renin antibody (ab57635)参考文献

ab57635 has not yet been referenced specifically in any publications.

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