概述

  • 产品名称Anti-Renalase抗体
    参阅全部 Renalase 一抗
  • 描述
    山羊多克隆抗体to Renalase
  • 特异性ab31291 recognises renalase.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-VSIDNKKRNI

    , corresponding to Internal sequence amino acids 224-234 of Human Renalase

  • 阳性对照
    • Human or mouse kidney lysate

性能

应用

Our Abpromise guarantee covers the use of ab31291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/32000.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.

In paraffin embedded Human Kidney shows vesicular staining in the epithelial cells of the CT.

靶标

  • 功能Probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. Degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis (By similarity). According to PubMed:17385068, is unlikely that renalase has physiologically relevant catecholamine-oxidizing activity.
  • 组织特异性Secreted into the blood by the kidney. Highly expressed in the kidney, expressed at lower level in heart, skeletal muscle and small intestine. Its plasma concentration is markedly reduced in patients with end-stage renal disease, as compared with healthy subjects.
  • 序列相似性Belongs to the renalase family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6530404N21Rik antibody
    • AI452315 antibody
    • AW060440 antibody
    • C10orf59 antibody
    • Chromosome 10 open reading frame 59 antibody
    • FLJ11218 antibody
    • HGNC:25641 antibody
    • Hypothetical protein LOC55328 antibody
    • MAO C antibody
    • MAO-C antibody
    • mMAO C antibody
    • Monoamine oxidase C antibody
    • Monoamine oxidase-C antibody
    • Renalase antibody
    • Renalase FAD dependent amine oxidase antibody
    • RNLS antibody
    • RNLS_HUMAN antibody
    see all

Anti-Renalase antibody 图像

  • In paraffin embedded Human Kidney using ab31291 it shows vesicular staining in the epithelial cells of the CT

  • Anti-Renalase antibody (ab31291) at 1 µg/ml (1 hour incubation) + Human Kidney lysate (35µg protein in RIPA buffer)
    Developed using the ECL technique

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • ab31291 (3µg/ml) staining Human Kidney by IHC-P. Microwaved antigen retrieval with citrate buffer (pH 6), HRP-staining.

Anti-Renalase antibody (ab31291)参考文献

This product has been referenced in:
  • Lee HT  et al. Renalase protects against ischemic AKI. J Am Soc Nephrol 24:445-55 (2013). WB ; Mouse . Read more (PubMed: 23393318) »
  • Pandini V  et al. Synthesis of human renalase1 in Escherichia coli and its purification as a FAD-containing holoprotein. Protein Expr Purif 72:244-53 (2010). WB ; Human . Read more (PubMed: 20302943) »

See all 2 Publications for this product

Product Wall

Thanks for your reply.

I think it will be very important, based on my knowledge of the situation, to get some specific details from the authors of the paper whose experiment you wish to reproduce. I suggest sending another email within a day ...

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Regarding your enquiry, I have spoken with my colleague John who you spoke with on August 9. He mentioned advising you to contact the authors of the study you are attempting to duplicate. I just wanted to know if you have heard from them yet ...

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Thank you for your email.

Yes, with the peptide you can make the standard curve.

When using unknown samples, you would just coat the well with the samples, but not the peptide. The peptide would be in separate wells. Also, try seve...

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Thank you for contacting us.

ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide. Please find below the protocol that was used, inc...

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Thank you for your enquiry. Unfortunately we do not provide the standard protein. This product was tested in peptide-ELISA, with the immunizing peptide bound to the plate. I am sorry I can't be of more assistance in this occasion. If there is a...

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Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab31291 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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Thank you for your enquiry. I have copied the ELISA protocol below: Peptide ELISA - Plates coated with immunising peptide at 1ug/ml, 4C overnight - Plates blocked blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05%Tween-20) for 1 hr at room te...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"