概述

  • 产品名称
    Anti-RELM alpha抗体
    参阅全部 RELM alpha 一抗
  • 描述
    兔多克隆抗体to RELM alpha
  • 经测试应用
    适用于: IHC-P, IHC-Fr, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length protein (Mouse)

性能

  • 形式
    Lyophilised:Reconstitute in sterile water to 0.2 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.

靶标

  • 功能
    Probable hormone.
  • 组织特异性
    Highest levels in adipose tissue.
  • 序列相似性
    Belongs to the resistin/FIZZ family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cysteine-rich secreted protein A12-gamma antibody
    • Cysteine-rich secreted protein FIZZ1 antibody
    • FIZZ1 antibody
    • Parasite-induced macrophage novel gene 1 protein antibody
    • RELMalpha antibody
    • Resistin like alpha antibody
    • Resistin-like alpha antibody
    • RETNA_MOUSE antibody
    • Retnla antibody
    see all

Anti-RELM alpha antibody 图像

  • ab39626 staining RELM alpha in Mouse cecum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (4 ng/ml) overnight at 4°C. A Cy3-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.

  • ab39626 at 4 ng/ml staining murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse) by IHC-P. The primary antibody was incubated overnight at 4˚C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit IgG.  Heat induced antigen retrieval with a 100mM Citric Acid was used.
  • ab39626 staining RELM alpha in murine liver tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde and then blocked using 5% BSA for 30 minutes. Samples were then incubated with ab39626 at a 1/20 dilution for 1 hour. The secondary used was a biotinylated goat anti-rabbit IgG used at a 1/200 dilution.

    See Abreview

Anti-RELM alpha antibody (ab39626)参考文献

This product has been referenced in:
  • Plastira I  et al. 1-Oleyl-lysophosphatidic acid (LPA) promotes polarization of BV-2 and primary murine microglia towards an M1-like phenotype. J Neuroinflammation 13:205 (2016). Read more (PubMed: 27565558) »
  • Braun GS  et al. IL-6 Trans-Signaling Drives Murine Crescentic GN. J Am Soc Nephrol 27:132-42 (2016). Read more (PubMed: 26041841) »

See all 13 Publications for this product

Product Wall

We have not suggested a positive control for ab39626. However, RELM alpha is predominantly found in adipose tissue therefore I could suggest Adipose (Mouse) Whole Cell Lysate - normal tissue (ab29417) as a positive control.

Thank you very much for contacting us with your enquiry.

I apologize for the delay getting this SDS for you. There was an IT issue with the PDF on the website, but this has now been corrected. I have also attached the PDF version for you. Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheets and for the inconvenience t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Perineal adipose tissue)
Specification
Perineal adipose tissue
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Mar 20 2012

Thank you for contacting us and your interest in our products.


As discussed over the phone ab8918isamouse monoclonaland canbe used with an anti-mouse IgG secondary antibody.


ab39626 and ab9969 are bothrabbit polyclonals ...

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Thank you for contacting us. The complete datasheet for each product can be found online. For ab39626 it is: http://www.abcam.com/RELM-alpha-antibody-ab39626.html There is also a button to create a pdf-file. I have attached this file to this email. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jun 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (bone marrow derived macrophages)
Loading amount
40 µg
Specification
bone marrow derived macrophages
Treatment
DMSO and IL-4 10 ng/ml
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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