Anti-Rel B (phospho S573)抗体(ab47366)

概述

  • 产品名称Anti-Rel B (phospho S573)抗体
    参阅全部 Rel B 一抗
  • 描述
    兔多克隆抗体to Rel B (phospho S573)
  • 特异性ab47366 detects endogenous levels of Rel B only when phosphorylated at serine 573.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Human Rel B (phospho S573). Synthesized peptide derived from human RelB around the phosphorylation site of Ser573.
    Database link: Q01201

  • 阳性对照
    • LoVo and RAW 264.7 cells, breast carcinoma tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab47366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
  • 序列相似性Contains 1 RHD (Rel-like) domain.
  • 结构域Both N- and C-terminal domains are required for transcriptional activation.
  • 翻译后修饰Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.
  • 细胞定位Nucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • I REL antibody
    • I-Rel antibody
    • IREL antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
    • relB antibody
    • RELB_HUMAN antibody
    • Reticuloendotheliosis viral oncogene homolog B antibody
    • Transcription factor Rel B antibody
    • Transcription factor RelB antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog B antibody
    • v rel reticuloendotheliosis viral oncogene homolog B antibody
    see all

Anti-Rel B (phospho S573) antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47366. Image on the left is tissue untreated with immunising peptide; image on the right is tissue treated with immunising peptide.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47366. Image on the left is tissue untreated with immunising peptide; image on the right is tissue treated with immunising peptide.
  • All lanes : Anti-Rel B (phospho S573) antibody (ab47366) at 1/500 dilution

    Lane 1 : LoVo (Human colorectal adenocarcinoma cell line) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate with phospho peptide


    Predicted band size : 62 kDa

Anti-Rel B (phospho S573) antibody (ab47366)参考文献

ab47366 has not yet been referenced specifically in any publications.

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