Anti-REG4抗体[MM0524-9B21] (ab89917)

概述

  • 产品名称Anti-REG4抗体[MM0524-9B21]
    参阅全部 REG4 一抗
  • 描述
    小鼠单克隆抗体[MM0524-9B21] to REG4
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human REG4 protein.

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human colon Adenocarcinoma.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明The IgG fraction of culture supernatant was purified by protein G affinity chromatography and 0.2 µm filtered.
  • 克隆单克隆
  • 克隆编号MM0524-9B21
  • 同种型IgG2
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 18 kDa.

靶标

  • 功能May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
  • 组织特异性Highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas and small intestine. Weakly expressed in normal colon and stomach. Strongly expressed in most colorectal tumors than in normal colon. Preferentially expressed in mucinous tumors and in some cases neuro-endocrine tumors. Expressed in mucus-secreting cells and enterocyte-like cells. In small intestine expressed at the basal perinuclear zone of goblet cells.
  • 序列相似性Contains 1 C-type lectin domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gastrointestinal secretory protein antibody
    • GISP antibody
    • Reg IV antibody
    • REG like protein antibody
    • REG-4 antibody
    • REG-like protein antibody
    • Reg4 antibody
    • REG4_HUMAN antibody
    • Regenerating gene type IV antibody
    • Regenerating islet derived family member 4 antibody
    • Regenerating islet derived protein 4 precursor antibody
    • Regenerating islet-derived protein 4 antibody
    • Regenerating islet-derived protein IV antibody
    • REGIV antibody
    • RELP antibody
    see all

Anti-REG4 antibody [MM0524-9B21] 图像

  • IHC image of REG4 staining in Human colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89917, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-REG4 antibody [MM0524-9B21] (ab89917)参考文献

ab89917 has not yet been referenced specifically in any publications.

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