All lanes : Anti-RED antibody (ab85965) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique
Predicted band size : 66 kDa
Exposure time : 10 seconds
Immunoprecipitation - RED antibody (ab85965)
1mg HeLa whole cell lysate was immunoprecipitated using 3µg ab85965. 20% of IP was loaded per lane and probed with with ab85965 at 1 µg/ml (lane 1) and with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 10 seconds.