Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region within N terminal amino acids 1 - 50 (MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVF V) of Human RECQL5 (NP_004250.4).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7.0-8.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
Use at 5-15 µg/mg of lysate.
May have an important role in DNA metabolism.
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Cytoplasm and Nucleus > nucleoplasm.
Information by UniProt
ATP-dependent DNA helicase Q5 antibody
DNA helicase antibody
DNA helicase, RecQ like type 5 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (ab91422)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RECQL5 with ab91422 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-RECQL5 antibody (ab91422)
All lanes : Anti-RECQL5 antibody (ab91422) at 0.1 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Predicted band size: 109 kDa
Immunoprecipitation - Anti-RECQL5 antibody (ab91422)
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91422.
20% of the immunoprecipitate was loaded per lane, and probed with ab91422 at 1µg/ml (lane 1) or a control IgG (lane2). Detection: chemoluminescence with an exposure time of 10 seconds.
This product has been referenced in:
et al. Altered RECQL5 expression in urothelial bladder carcinoma increases cellular proliferation and makes RECQL5 helicase activity a novel target for chemotherapy. Oncotarget 7:76140-76150 (2016).
Read more (PubMed: 27764811) »
et al. Mutation of cancer driver MLL2 results in transcription stress and genome instability. Genes Dev 30:408-20 (2016).
Read more (PubMed: 26883360) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"