概述

  • 产品名称Anti-RECQ1抗体
    参阅全部 RECQ1 一抗
  • 描述
    兔多克隆抗体to RECQ1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human RECQ1.

    (Peptide available as ab25318.)

  • 阳性对照
    • This antibody gave a positive signal in HeLa whole cell lysate and HeLa nuclear lysate in Western blot, HeLa whole cell lysate in Immunofluorescence and Human Kidney tissue lysate in Immunohistochemistry.

性能

应用

Our Abpromise guarantee covers the use of ab22830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).

靶标

  • 相关性The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ATP dependent DNA helicase Q1 antibody
    • DNA helicase Q1 like antibody
    • DNA helicase RecQ like type 1 antibody
    • RECQ L1 antibody
    • RecQ protein like antibody
    • RecQ protein like 1 antibody
    • RECQL antibody
    • RECQL1 antibody
    see all

Anti-RECQ1 antibody 图像

  • All lanes : Anti-RECQ1 antibody (ab22830) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 ug
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 ug
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 ug with Human RECQ1 peptide (ab25318) at 1 µg/ml
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 ug with Human RECQ1 peptide (ab25318) at 1 µg/ml

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 28 kDa (possible cross reactivity),32-36 kDa (possible cleavage fragment,cross reactivity),48 kDa (possible cleavage fragment,cross reactivity).
  • ICC/IF image of ab22830 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22830, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    Panel A shows localisation of ab22830 to the nuclei, Panel B has the Alexa Fluor® 488 channel removed for comparison.

  • IHC image of ab22830 staining RECQ1 in Human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22830, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-RECQ1 antibody (ab22830)参考文献

ab22830 has not yet been referenced specifically in any publications.

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