Anti-Recoverin抗体[6A55CD6] (ab31928)

概述

  • 产品名称
    Anti-Recoverin抗体[6A55CD6]
    参阅全部 Recoverin 一抗
  • 描述
    小鼠单克隆抗体[6A55CD6] to Recoverin
  • 宿主
    Mouse
  • 经测试应用
    适用于: ELISA, ICC/IF, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Cow
  • 免疫原

    Recombinant full length protein of Human Recoverin.

  • 阳性对照
    • ab31928 gave a positive result in Mouse Retina Whole Cell Lysate.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab31928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 19361860
WB Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IHC-FoFr Use at an assay dependent concentration. See Abreview.

靶标

图片

  • All lanes : Anti-Recoverin antibody [6A55CD6] (ab31928) at 2 µg/ml

    Lane 1 : Mouse Retina Whole Cell Lysate at 10 µg
    Lane 2 : Human Retina Normal Tissue Lysate at 20 µg

    Secondary
    All lanes : Rabbit polyclonal to Mouse IgG H&L (HRP) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

  • ab31928 staining Recoverin in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in paraformaldehyde without permeation and blocking steps. The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/1000 was used as secondary.

    See Abreview

文献

This product has been referenced in:
  • Liu Y  et al. Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells. Int J Mol Med 40:1172-1184 (2017). Read more (PubMed: 28848998) »
  • Redenti S  et al. Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials 30:3405-14 (2009). ICC/IF ; Mouse . Read more (PubMed: 19361860) »

See all 2 Publications for this product

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cells from cornea)
Specification
cells from cornea
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 16 2010

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Jan 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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