Anti-Recoverin抗体[6A55CD6] (ab31928)


  • 产品名称Anti-Recoverin抗体[6A55CD6]
    参阅全部 Recoverin 一抗
  • 描述
    小鼠单克隆抗体[6A55CD6] to Recoverin
  • 经测试应用适用于: ELISA, ICC/IF, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Cow
  • 免疫原

    Recombinant full length protein of Human Recoverin.

  • 阳性对照
    • ab31928 gave a positive result in Mouse Retina Whole Cell Lysate.



Our Abpromise guarantee covers the use of ab31928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IHC-FoFr Use at an assay dependent concentration. See Abreview.


Anti-Recoverin antibody [6A55CD6] 图像

  • All lanes : Anti-Recoverin antibody [6A55CD6] (ab31928) at 2 µg/ml

    Lane 1 : Mouse Retina Whole Cell Lysate at 10 µg
    Lane 2 : Human Retina Normal Tissue Lysate at 20 µg

    Rabbit polyclonal to Mouse IgG H&L (HRP) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • ab31928 staining Recoverin in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in paraformaldehyde without permeation and blocking steps. The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/1000 was used as secondary.

    See Abreview

Anti-Recoverin antibody [6A55CD6] (ab31928)参考文献

This product has been referenced in:
  • Redenti S  et al. Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials 30:3405-14 (2009). ICC/IF ; Mouse . Read more (PubMed: 19361860) »

See 1 Publication for this product

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This product is Protein G purified.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cells from cornea)
Specification cells from cornea
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Sep 16 2010

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No

Dr. Sophie Pezet

Verified customer

提交于 Jan 28 2009