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Our Abpromise guarantee covers the use of ab31928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).|
|IHC-FoFr||Use at an assay dependent concentration. See Abreview.|
ab31928 staining Recoverin in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in paraformaldehyde without permeation and blocking steps. The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/1000 was used as secondary.
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