重组herpes simplex virus viral MIP2蛋白(ab50234)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Inhibition Assay, SDS-PAGE
描述
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产品名称
重组herpes simplex virus viral MIP2蛋白
参阅全部 viral MIP2 蛋白酶 -
生物活性
Determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0 µg-10.0 µg/ml of viral MIP-2 will inhibit 25 ng/ml of human MIP-1 alpha.
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纯度
> 95 % SDS-PAGE.
ab50234 purity is greater than 98% by SDS-PAGE and HPLC analyses. -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Herpes simplex virus -
序列
LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTA
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技术指标
Our Abpromise guarantee covers the use of ab50234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Inhibition Assay
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
常规信息
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别名
- Viral macrophage inflammatory protein II
- vMIP 1B
- vMIP II
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相关性
vMIP2 is a chemokine analog encoded by the human herpesvirus (HHV8) and is a potent in vitro antagonist of many chemokine receptors. In vivo vMIP2 has been shown to be a potent inhibitor of type 1 T-cell-mediated inflammation. Three chemokine-like proteins, vMIP-I, vMIP-II and vMIP-III are encoded within the HHV8 genome. Among human chemokines, vMIP2 is most closely related to MIP-1a, sharing approximately 41% amino acid sequence identity. The CC chemokine receptor (CCR) 8 belongs to the seven transmembrane-spanning receptor families and functionally responds to the eukaryotic CC chemokines I-309, MIP 1b and vMIPI and vMIP2. Both vMIP I and vMIP2 partially block HIV infection of peripheral blood mononuclear cells. vMIPI and vMIP2 are also highly angiogenic. Chemokines play a profound role in leukocyte trafficking and the development of adaptive immune responses. Perhaps due to their importance in host defense, viruses have adopted many of the hallmarks displayed by chemokines. One therapeutic strategy to prevent accumulation of pro-inflammatory immune cells is the use of specific chemokine receptor antagonists. An interesting and promising candidate in this context is the viral antagonist vMIP2 as this molecule acts on a broad spectrum of chemokine receptors. -
细胞定位
Secreted
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab50234 尚未被引用在任何文献中。