Recombinant T4 DNA Ligase protein (ab68649)

概述

  • 产品名称Recombinant T4 DNA Ligase protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Escherichia coli

技术指标

Our Abpromise guarantee covers the use of ab68649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性167,000 U/ml. Unit Defenition: One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of lambda DNA (5´ DNA termini concentration of 0.12 µM, 300µg/ml) in a total reaction volume of 20 µl in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer: (50mM Tris-HCl (pH 7.8 at 25°C), 10mM Magnesium chloride, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 µM in 5´ termini)).
  • 应用

    Functional Studies

  • 形式Liquid
  • 补充说明T4 DNA Ligase can be inactivated by incubation at 65°C for 10 minutes. Optimal ligation occurs at 16°C. To dilute T4 DNA Ligase that will subsequently be stored at –20°C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used. T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM. Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50µM.


    This protein can be used in Cloning of restriction fragments and joining linkers and adapters to blunt-ended DNA.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 200µg/ml BSA, 50mM Potassium chloride, 10mM Tris HCl, 1mM DTT, 0.1mM EDTA, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Recombinant T4 DNA Ligase protein (ab68649)参考文献

ab68649 has not yet been referenced specifically in any publications.

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