Recombinant Streptavidin protein (ab78833)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 序列MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
    • 分子量17 kDa including tags
    • 氨基酸25 to 183
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab78833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab78833 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM Tris, pH 7.5

常规信息

  • 别名
    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • 相关性Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • 细胞定位Cytoplasmic

Recombinant Streptavidin protein 图像

  • 15% SDS-PAGE showing ab78833 at approximately 17kDa (3µg).

Recombinant Streptavidin protein (ab78833)参考文献

This product has been referenced in:
  • Qin GW et al. Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications Journal of Nanoparticle Research 13:45-51 (2010). ELISA . Read more (PubMed: ) »

See 1 Publication for this product

Product Wall

We weren’t sure what your client means by “accessible”. If they’re asking whether it’s detectable with a His tag antibody, then in principle it should be. If they are asking whether it’s the cleavable form, regrettab...

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I can confirm that the His tag is functional in this peptide for complexing with Nickel. The Met and Val in front of the His tag helps the His tag for proper configuration and has no impact on the protein’s functionality. I hope this information is...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"