The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity: Greater than 95% binding activity to human IgG.
% SDS-PAGE. Staphylococal Protein A was purified by proprietary chromatographic techniques.
Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE, Silver Stained.
Note: A specific purification process was used to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and the protein is devoid of bacterial contaminants found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin).
This protein was produced by expressing a modified protein A gene in E.coli.
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Shipped at 4°C. Store at +4°C.
Constituents: 1X PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
IgG binding protein A
Immunoglobulin G binding protein A
S.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence.
Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Secreted; cell wall.
has not yet been referenced specifically in any publications.