Recombinant S. cerevisiae Thioredoxin / TRX protein (ab82731)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Saccharomyces cerevisiae

技术指标

Our Abpromise guarantee covers the use of ab82731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity : 32.7 U/mg. One unit will cause a deltaA650 of 1.0 in 1min at 25°C in the insulin reduction assay.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20mM HEPES, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in 20mM HEPES pH 7.0, Packaging size : 0.5 mg

常规信息

  • 别名
    • ADF
    • ATL derived factor
    • ATL-derived factor
    • DKFZp686B1993
    • MGC61975
    • SASP
    • Surface associated sulphydryl protein
    • Surface-associated sulphydryl protein
    • testicular tissue protein Li 199
    • THIO_HUMAN
    • Thioredoxin
    • thioredoxin delta 3
    • TRDX
    • Trx
    • TRX 1
    • TRX1
    • TXN
    • TXN delta 3
    • TXN protein
    • zgc:92903
    see all
  • 功能Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • 序列相似性Belongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • 翻译后修饰In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • 细胞定位Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt

Recombinant S. cerevisiae Thioredoxin / TRX protein 图像

  • ab82731 at 1µg on SDS-PAGE.

Recombinant S. cerevisiae Thioredoxin / TRX protein (ab82731)参考文献

ab82731 has not yet been referenced specifically in any publications.

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