重组S. cerevisiae GAL4蛋白(ab81879)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组S. cerevisiae GAL4蛋白
参阅全部 GAL4 蛋白酶 -
纯度
> 95 % SDS-PAGE.
ab81879 is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter. -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Saccharomyces cerevisiae -
氨基酸
1 to 147
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描述
重组S. cerevisiae GAL4蛋白
技术指标
Our Abpromise guarantee covers the use of ab81879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3`. A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an {alpha}-helix, to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
常规信息
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别名
- Antigen NY CO 27
- Antigen NY-CO-27
- Antigen NYCO27
see all -
功能
Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions. -
序列相似性
Contains 2 galectin domains. -
结构域
Contains two homologous but distinct carbohydrate-binding domains. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab81879 尚未被引用在任何文献中。