Recombinant Rubella virus E1 protein (ab68498)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 序列The E.Coli derived recombinant protein contains the Rubella Virus E1 regions, 157-176, 374-390, 213-239 amino acids.
    • 额外的序列信息The protein is fused to a GST-tag.

技术指标

Our Abpromise guarantee covers the use of ab68498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性ab68498 is immunoreactive with sera of Rubella Virus infected individuals.
  • 应用

    Western blot

    ELISA

  • 纯度> 95 % SDS-PAGE.
    ab68498 is >95% pure as determined by 10% PAGE (coomassie staining), purified by a proprietary chromatographic technique.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM imidazol, 8M Urea, 0.3M Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • E1
    • E1 envelope glycoprotein
    • Glycoprotein E1
    • Spike glycoprotein E1
  • 相关性Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It is an enveloped (toga=cloak), non-segmented, positive sense, RNA virus and replicates in the cytoplasm. It consists of 3 structural proteins; E1,E2 membrane bound glycoproteins, and C capsid protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and endocytosis, acidification of the endosome induces dissociation of E1/E2 heterodimer and trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in the cytoplasm after cell and viral membrane fusion. The E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.

Recombinant Rubella virus E1 protein (ab68498)参考文献

ab68498 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your phone call yesterday and for your question about the E1 protein ab68498. I have been in touch with the lab and was told that it is a dimer around 80 kDa.  I hope this information is helpful, but please let me know if yo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"