The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. The recombinant RFP (Red Fluorescence Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence of the protein. Endotoxin was removed for in vivo injection or cell culture applications. Endotoxin level equal to or less than 0.1 ng/µg.
This protein (DSRed) is suitable as control reagent for RFP expression studies or as a labeling reagent.
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Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Reconstitute with 100 µl dH2O to 1mg/ml
GFP like chromoprotein
red fluorescent protein
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Recombinant RFP protein 图像
SDS-PAGE - Recombinant RFP protein (ab51993)
ab51993 loaded under reducing conditions and stained with coomassive blue.
Recombinant RFP protein (ab51993)参考文献
This product has been referenced in:
Dufour YS et al. Direct Correlation between Motile Behavior and Protein Abundance in Single Cells. PLoS Comput Biol12:e1005041 (2016).
Read more (PubMed: 27599206) »