Recombinant大鼠VEGFC protein (ab52146)

概述

描述

  • 性质Recombinant
  • 来源Insect cells
  • 氨基酸序列
    • AccessionO35757
    • 种属Rat
    • 序列DTVKLAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKP PSVSVYRCGGCCNSEGLQCMNTSTGYLSKTLFEITVPLSQGPKPVTISFA NHTSCRCMSKLDVYRQVHSIIHHHHHH
    • 氨基酸101 to 221
    • 修饰mutated C152 S
    • 标签His tag C-Terminus

技术指标

Our Abpromise guarantee covers the use of ab52146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    This product is analog to the human VEGF-C156S mutant and only active toward VEGFR-3/FLT -4 but, unlike wild type VEGF-C, is unable to bind to and to activate signalling through VEGFR-2/KDR.

    Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT -4 cells). The ED50 for this effect is typically 150-300 ng/ml. Inactive in the vascular permeability assay.

  • 应用

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.
    Purity >90% by SDS-PAGE and visualised by silver stain. Product is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGFC by a Ser residue.
  • 形式Lyophilised
  • 补充说明Source: Insect cells.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, BSA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitution: The lyophilised VEGFC is soluble in water and most aqueous buffers. The lyophilised VEGFC should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.

常规信息

  • 别名
    • Flt 4L
    • Flt4 ligand
    • FLT4 ligand DHM
    • Flt4-L
    • Flt4L
    • Vascular endothelial growth factor C
    • Vascular endothelial growth factor related protein
    • Vascular endothelial growth factor-related protein
    • VEGF C
    • VEGF-C
    • Vegfc
    • VEGFC_HUMAN
    • VRP
    see all
  • 功能Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • 组织特异性Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 翻译后修饰Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant rat VEGFC protein (ab52146)参考文献

ab52146 has not yet been referenced specifically in any publications.

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