重组大鼠PDGF AA蛋白(ab202796)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, HPLC
描述
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产品名称
重组大鼠PDGF AA蛋白
参阅全部 PDGF AA 蛋白酶 -
生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/mL, corresponding to a specific activity of > 2.0 × 105 IU/mg.
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纯度
> 98 % SDS-PAGE.
> 98 % by HPLC analysis. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Rat -
序列
MSIEEAIPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNT SSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATSNLN PDHREEETDVR -
预测分子量
25 kDa -
氨基酸
87 to 196 -
额外的序列信息
This product is the mature full length protein from aa 87 to 196. The signal peptide and propeptide are not included. Disulfide-linked homodimeric protein.
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技术指标
Our Abpromise guarantee covers the use of ab202796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
HPLC
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: 0.1% Trifluoroacetic acid
Lyophilised from a 0.2 µm filtered concentrated solution containing 30 % Acetonitrile.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20°C to -70°C. Further dilutions should be made in appropriate buffered solutions.
常规信息
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别名
- PDGF A
- PDGF A chain
- PDGF subunit A
see all -
功能
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
结构域
The long form contains a basic insert which acts as a cell retention signal. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab202796 被引用在 1 文献中.
- Dong Z et al. Identification lncRNA LOC102551149/miR-23a-5p pathway in hepatic fibrosis. Eur J Clin Invest 50:e13243 (2020). PubMed: 32306379