Recombinant大鼠nNOS (neuronal) protein (ab59110)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Rat

技术指标

Our Abpromise guarantee covers the use of ab59110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: >200 units/mg protein. The activity of recombinant rat nNOS (neuronal) is determined by an oxyhemoglobin assay that measures the reaction of nitric oxide with oxyhemoglobin to yield methemoglobin. One unit of enzyme produces 1 nmole of nitric oxide per minute at 37°C in 50 mM HEPES, pH 7.4, containing 5µM oxyhemoglobin, 1 mM CaCl2, 20µg/ml calmodulin, 0.1 mM NADPH, 50µM arginine, 12µM tetrahydrobiopterin, and 170µM DTT. nNOS (neuronal) is calcium/ calmodulin dependent and has a Km for arginine of approximately 2µM.
  • 纯度> 95 % SDS-PAGE.
    Produced in SF9 cells from a Baculovirus over-expression system. Purity of the protein is >95%.
  • 形式Liquid
  • 补充说明The enzyme loses approximately 40% of its activity during a single freeze thaw cycle. During use, keep the solution on ice at all times since the enzyme is unstable at higher temperatures.


    Specific Activity: >300 units/mg protein. The activity of recombinant rat nNOS (neuronal) is determined by an oxyhemoglobin assay that measures the reaction of nitric oxide with oxyhemoglobin to yield methemoglobin. One unit of enzyme produces 1 nmole of nitric oxide per minute at 37°C in 50 mM HEPES, pH 7.4, containing 5µM oxyhemoglobin, 1 mM CaCl2, 20µg/ml calmodulin, 0.1 mM NADPH, 50µM arginine, 12µM tetrahydrobiopterin, and 170µM DTT. nNOS (neuronal) is calcium/ calmodulin dependent and has a Km for arginine of approximately 2µM.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 1µM Tetrahydrobiopterin, 20% Glycerol, 50mM HEPES, 100mM Sodium chloride, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 2310005C01Rik
    • BNOS
    • Constitutive NOS
    • EC 1.14.13.39
    • IHPS 1
    • IHPS1
    • N-NOS
    • NC-NOS
    • neuronal Nitric Oxide Synthase
    • Neuronal NOS
    • Nitric oxide synthase , neuronal, included
    • Nitric oxide synthase 1
    • Nitric oxide synthase 1 (neuronal)
    • Nitric oxide synthase, brain
    • Nitric oxide synthase, penile neuronal, included
    • NNOS
    • NO
    • NOS
    • NOS 1
    • NOS type I
    • NOS-I
    • NOS1
    • NOS1_HUMAN
    • Peptidyl-cysteine S-nitrosylase NOS1
    see all
  • 功能Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • 组织特异性Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • 序列相似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • 翻译后修饰Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • 细胞定位Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt

Recombinant rat nNOS (neuronal) protein (ab59110)参考文献

ab59110 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.  I was informed that this lot (GR27013) of ab59110 contains 350 units/mg of protein and 1000 μg of protein per ml.  I hope this information is helpful to you. Please do not hesitate to contact us if you need any mor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"