重组大鼠IL-17A + IL-17F蛋白(ab109681)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
描述
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产品名称
重组大鼠IL-17A + IL-17F蛋白 -
生物活性
The ED50 of ab109681, as determined by its ability to induce IL-6 production in NIH-3T3 cells, is 7.28ng/mL. -
纯度
> 98 % SDS-PAGE.
Purity Greater than 98% determined by reducing and non-reducing SDS-PAGE. -
内毒素水平
< 0.100 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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氨基酸序列 1
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种属
Rat -
序列
MAVLIPQSSVCPNAEANNFLQNVKVNLKVLNSLSSKASSRRPSDYLNRST SPWTLSRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILV LKREPEKCPFTFRVEKMLVGVGCTCVSSIVRHAS -
预测分子量
15 kDa -
氨基酸
18 to 150
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氨基酸序列 2
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种属
Rat -
序列
MAVLIPQSSVCPNAEANNFLQNVKVNLKVLNSLSSKASSRRPSDYLNRST SPWTLSRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILV LKREPEKCPFTFRVEKMLVGVGCTCVSSIVRHAS -
预测分子量
15 kDa -
氨基酸
29 to 161
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技术指标
Our Abpromise guarantee covers the use of ab109681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
HPLC
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. The formulation is from 0.02% TFA before product is lyophilized
常规信息
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别名
- Ctla 8
- CTLA8
- Cytokine ML1
see all -
相关性
IL-17F stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. IL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
细胞定位
Secreted
图片
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SDS PAGE analysis of ab109681 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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ab109681 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Rat IL-17AF is a heterodimer with a predicted total MW of 30.7 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab109681 尚未被引用在任何文献中。