Recombinant大鼠Hsp60 protein (ab92365)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Rat

技术指标

Our Abpromise guarantee covers the use of ab92365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    ATPase Activity Assay: Positive
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度
    > 90 % SDS-PAGE.
    >90 % pure as determined by SDS-PAGE and Western blot analyses. This protein does not contain E. coli GroEL as demonstrated by western blot analysis.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, 150mM Sodium chloride, 1mM DTT, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 60 kDa chaperonin
    • 60 kDa heat shock protein, mitochondrial
    • CH60_HUMAN
    • Chaperonin 60
    • Chaperonin, 60-KD
    • CPN60
    • fa04a05
    • GROEL
    • heat shock 60kDa protein 1 (chaperonin)
    • Heat shock protein 1 (chaperonin)
    • Heat shock protein 60
    • Heat shock protein 65
    • heat shock protein family D (Hsp60) member 1
    • HLD4
    • Hsp 60
    • HSP 65
    • HSP-60
    • HSP60
    • HSP65
    • HSPD1
    • HuCHA60
    • Mitochondrial matrix protein P1
    • P60 lymphocyte protein
    • short heat shock protein 60 Hsp60s1
    • SPG13
    see all
  • 功能
    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • 疾病相关
    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • 序列相似性
    Belongs to the chaperonin (HSP60) family.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt

图片

  • SDS-PAGE Analysis:
    Lane 1: Molecular weight markers
    Lane 2: ab92365 at 2.0 µg
  • All lanes : Hsp60 monoclonal antibody at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : ab92365 at 0.1 µg
    Lane 3 : E. coli GroEL Protein at 0.1 µg
  • All lanes : GroEL monoclonal antibody at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : ab92365 at 0.1 µg
    Lane 3 : E. coli GroEL Protein at 0.1 µg

文献

ab92365 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam about HSP60 protein endotoxin levels. We have not performed endotoxin testing on ab92365 but we have tested ab113192.  For ab113192, the endotoxin level is >500EU/mg purified protein (LAL test).   If there is anything els...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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