Recombinant大鼠G protein alpha (mutated L227 + N295) (ab90406)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Rat

技术指标

Our Abpromise guarantee covers the use of ab90406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

  • 形式Liquid
  • 补充说明


    GTP-binding possesses a highly conserved aspartate residue in the NKXD motif that is critical for high-affinity interaction with GTP. In almost all GTP-binding proteins so far, the D/N-mutation switches base-specifity from guanine to xanthine. Whereas the exchange of Asp295 to Asn295 leads to inactive mutants of Galpha-subunits, an additional Q/L-mutation in the catalytic site (Gln227 to Leu227) rescues protein function and induces xanthine nucleotide-specifity.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 75mM Tris HCl, 12.5mM Magnesium chloride, 1mM EDTA, pH 7.4

常规信息

  • 别名
    • Adenylate cyclase stimulating G alpha protein
    • AHO
    • C20orf45
    • Extra large alphas protein
    • GNAS complex locus
    • GNAS1
    • GPSA
    • GSA
    • GSP
    • Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1
    • Guanine nucleotide binding protein G(s) alpha subunit
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas
    • guanine nucleotide regulatory protein
    • NESP
    • Neuroendocrine secretory protein
    • PHP1A
    • PHP1B
    • PHP1C
    • POH
    • RP4-543J19.4
    • Secretogranin VI
    • XLalphas
    see all
  • 相关性GTP-binding proteins (G-proteins)are a family of heterotrimeric proteins that play a critical role in signal transduction by coupling cell surface, 7-transmembrane domain receptors to intracellular signaling pathways including second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth and differentiation. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit.
  • 细胞定位Cell Membrane

Recombinant Rat G protein alpha (mutated L227 + N295) (ab90406)参考文献

ab90406 has not yet been referenced specifically in any publications.

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