The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性Specific activity =5 x 106 units/mg.
内毒素水平< 0.100 Eu/µg
% SDS-PAGE. Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
补充说明Reconstituted FGF basic protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C.
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Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Basic fibroblast growth factor
Basic fibroblast growth factor bFGF
Fibroblast growth factor 2
Fibroblast growth factor 2 (basic)
Fibroblast growth factor, basic
Heparin binding growth factor 2 precursor
Heparin-binding growth factor 2
功能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
组织特异性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
序列相似性Belongs to the heparin-binding growth factors family.
翻译后修饰Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
细胞定位Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.