Recombinant大鼠EGF protein (ab123750)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP07522
    • 种属Rat
    • 序列NSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWW KLR
    • 氨基酸974 to 1026

技术指标

Our Abpromise guarantee covers the use of ab123750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 1 x 107units/mg.
  • 应用

    HPLC

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab123750 is purified by proprietary chromatographic techniques. The Rat EGF Purity is greater than 98% as determined by HPLC and SDS-PAGE.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20ºC.

    pH: 7.40
    Constituent: 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Centrifuge the vial prior to opening. Reconstitute in sterile H2O to a concentration = 100 µg/ml. This solution can then be diluted into other aqueous buffer.

常规信息

  • 别名
    • Beta urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all
  • 功能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 组织特异性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 疾病相关Hypomagnesemia 4
  • 序列相似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻译后修饰O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant rat EGF protein (ab123750)参考文献

ab123750 has not yet been referenced specifically in any publications.

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