Recombinant大鼠CREB protein (ab43602)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Rat
    • 氨基酸1 to 280

技术指标

Our Abpromise guarantee covers the use of ab43602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

常规信息

  • 别名
    • Active transcription factor CREB
    • cAMP response element binding protein
    • cAMP response element binding protein 1
    • cAMP responsive element binding protein 1
    • cAMP-responsive element-binding protein 1
    • CREB
    • CREB-1
    • CREB1
    • CREB1_HUMAN
    • Cyclic AMP-responsive element-binding protein 1
    • MGC9284
    • OTTHUMP00000163864
    • OTTHUMP00000163865
    • OTTHUMP00000206660
    • OTTHUMP00000206662
    • OTTHUMP00000206667
    • Transactivator protein
    see all
  • 功能This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
  • 疾病相关Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
  • 序列相似性Belongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • 翻译后修饰Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Rat CREB protein (ab43602)参考文献

ab43602 has not yet been referenced specifically in any publications.

Product Wall

Ab43602 CREB protein (Tagged) has been created with an N-terminal GST tag. The approximate molecular weight of this is 59 kDa. The purity of this CREB protein was determined to be >70% by densitometry.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"