Recombinant大鼠Biliverdin Reductase protein (ab91622)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Rat

技术指标

Our Abpromise guarantee covers the use of ab91622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Biliverdin Reductase Assay: Positive (> 180 units/mg) 1 unit of biliverdin reductase will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    Purified by Multi-Step Chromatography. > 95% pure as determined by SDS-PAGE and Western Blot analysis.
  • 形式Liquid
  • 补充说明Biliverdin Reductase Assay: Positive (> 180 units/mg) 1 unit of biliverdin reductase will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, 5mM DTT, 1mM EDTA, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • BIEA_HUMAN
    • Biliverdin IX alpha reductase
    • Biliverdin reductase A
    • Biliverdin-IX alpha-reductase
    • BLVR
    • BLVR A
    • Blvra
    • BVR
    • BVR A
    • BVRA
    • Zinc metalloprotein
    see all
  • 功能Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor.
  • 组织特异性Liver.
  • 通路Porphyrin-containing compound metabolism; protoheme degradation.
  • 疾病相关Hyperbiliverdinemia
  • 序列相似性Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant rat Biliverdin Reductase protein 图像

  • SDS-PAGE Analysis:
    Lane 1: Molecular weight marker
    Lane 2: 0.5 µg ab91622
    Lane 3: 1.0 µg ab91622
    Lane 4: 2.0 µg ab91622
    Lane 5: 5.0 µg ab91622
  • All lanes : Biliverdin Reductase Polyclonal Antibody

    Lane 1 : Molecular weight markers
    Lane 2 : ab91622 at 0.1 µg
  • Biliverdin Reductase Activity: 1 µg of ab91622 was incubated with biliverdin in the presence of NADPH at pH 8.5, 37°C. Product formation was monitored with a spectrometer at 450 nm.

Recombinant rat Biliverdin Reductase protein (ab91622)参考文献

ab91622 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab91622.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"