重组蛋白G (ab49807)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 分子量26 kDa including tags
    • 氨基酸190 to 384
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab49807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Affinity purification

  • 纯度> 95 % SDS-PAGE.
    >98% by SDS-PAGE and HPLC analyses. The albumin binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
  • 形式Lyophilised
  • 补充说明


    Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies.

    This protein contains only IgG binding domains.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is <0.1 EU/mg of Protein G.

常规信息

  • 别名
    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • 相关性Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • 细胞定位Cell Wall and Secreted

Recombinant Protein G (ab49807)参考文献

ab49807 has not yet been referenced specifically in any publications.

Product Wall

The protein is resuspended in water to a concentration of 5 mg/ml.



Ma collègue est actuellement en contact avec un laboratoire américain pour la protéine G entière sans tag.

Elle m'a dit qu'elle espérait un retour dans la semaine mais n'oublions pas que jeudi et ve...

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J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
- une protéine G (full length sans tag) en compl&ea...

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Je viens d'être informé que:

1) les deux protéines A et G His-taggées ab49807 et ab52953 ne sont malheureusement pas disponibles dans la forme sans tag.

2) nous avons ou pouvons ajouter à notre catalo...

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Je suis toujours en attente d'une réponse pour une proposition de protéine His-tag pouvant fixer le fer ou un métal lourd.

Concernant les protéines A et G, nous avons ab49807 (protéine G, acides aminés...

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Voici comme convenu la réponse du laboratoire ainsi que le séquence de ab49807:

ab49807 : EL DKYGVSDYHK NLINNAKTVE GVKDLQAQVV ESAKKARISE ATDGLSDFLK SQTPAEDTVK SIELAEAKVL ANRELDKYGV SDYYKNLINN AKTVEGVKAL IDEILAALPK TDTYKLILNG KTLK...

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I was forwarded your information by my colleague, and I'm happy to help out with your enquiries.

I'll be in touch with the manufacturing lab, and I can give you a call anytime this afternoon. Are you free around noon CDT?

Please let...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"