重组蛋白G (ab49807)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组蛋白G
参阅全部 Protein G 蛋白酶 -
纯度
> 95 % SDS-PAGE.
>98% by SDS-PAGE and HPLC analyses. The albumin binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Streptococcus -
序列
LDKYGVSDYHKNLINNAKTVEGVKDLQAQVVESAKKARISEATDGLSDFL KSQTPAEDTVKSIELAEAKVLANRELDKYGVSDYYKNLINNAKTVEGVKA LIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNG VDGE -
预测分子量
26 kDa including tags -
氨基酸
190 to 384 -
标签
His tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab49807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Affinity Purification
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形式
Lyophilized -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 6510 Protein G. 6510-1 is the same size as the 1 mg size of ab49807.
Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies.
This protein contains only IgG binding domains.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 10.269% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate
常规信息
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别名
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
see all -
相关性
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. -
细胞定位
Cell Wall and Secreted
图片
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Different amounts of Recombinant Protein G loaded under reducing conditions and stained with Coomassie Blue. The protein has a predicted molecular weight (MW) of ∼ 26.1 kDa. However it runs larger on a SDS-PAGE gel. SDS-PAGE (12%) of Recombinant Protein G:
Lane 1: MW Marker
Lane 2 : Protein G ( 2.2 µg)
Lane 3 : Protein G ( 4.4 µg)
Lane 4 : Protein G ( 8.8 µg)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab49807 被引用在 5 文献中.
- Yasunaga AB & Li ITS Quantification of fast molecular adhesion by fluorescence footprinting. Sci Adv 7:N/A (2021). PubMed: 34407937
- Korodi M et al. Reusable on-plate immunoprecipitation method with covalently immobilized antibodies on a protein G covered microtiter plate. J Immunol Methods 483:112812 (2020). PubMed: 32569597
- Park S et al. Force-dependent trans-endocytosis by breast cancer cells depletes costimulatory receptor CD80 and attenuates T cell activation. Biosens Bioelectron 165:112389 (2020). PubMed: 32729511
- Li IT et al. Mapping cell surface adhesion by rotation tracking and adhesion footprinting. Sci Rep 7:44502 (2017). PubMed: 28290531
- Wang X et al. Constructing modular and universal single molecule tension sensor using protein G to study mechano-sensitive receptors. Sci Rep 6:21584 (2016). PubMed: 26875524