重组蛋白A +蛋白G (ab52213)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组蛋白A +蛋白G -
生物活性
Protein A+G binds to all IgG subclasses (from various mammalian species). -
纯度
> 98 % SDS-PAGE. -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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标签
His tag N-Terminus -
额外的序列信息
The molecular weight including the His tag is 59.7 kDa Protein A: P02976 Protein G: P19909
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相关产品
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Positive Controls
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab52213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Affinity Purification
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形式
Lyophilized -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 6502 Protein A/G. 6502-1 is the same size as the 1 mg size of ab52213.
This product is a genetically engineered fusion protein that combines the IgG binding profiles of both Protein A and Protein G. Protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. The 6x His-tag on the N-terminus can be used for affinity purification or for protein A/G detection using an anti-His-tag antibody. Non-specific binding regions, including cell wall binding regions, albumin binding regions and other non-specific binding regions have been eliminated from this fusion protein A/G, to ensure the maximum specific IgG binding.
Protein A+G binds to all IgG subclasses (from various mammalian species).
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
复溶Reconstitute in water to a concentration of 5 mg/ml.
常规信息
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细胞定位
Protein A: Secreted; cell wall. Protein G: Cell Wall and Secreted
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab52213 被引用在 1 文献中.
- Ettrup KS et al. The anatomy of the Göttingen minipig hypothalamus. J Chem Neuroanat 39:151-65 (2010). PubMed: 20043984