Recombinant Pasteurella multocida toxin protein (ab63832)

概述

  • 产品名称Recombinant Pasteurella multocida toxin protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Escherichia coli

技术指标

Our Abpromise guarantee covers the use of ab63832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Induction of aggregation of Swiss 3T3 cells by incubation with PMT.
  • 应用

    SDS-PAGE

    Cellular activity

    Western blot

    ELISA

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • 补充说明

    ab63832 can be used for studies on the GTP trimer bound protein dependent signaling pathways.

    Induction of aggregation of Swiss 3T3 cells by incubation with PMT. Induction of aggregation of Swiss 3T3 cells by incubation with PMT.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    pH: 7.50
    Constituents: 0.079% Tris HCl, 50% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Dermonecrotic toxin
    • DNT
    • Mitogenic toxin
    • PMT
    • ToxA
    see all
  • 相关性Pasteurella multocida toxin (PMT) is produced by a gram-negative bacillus, Pasteurella multocida. PMT activates the Gq and G12/13 dependent signaling pathways. Gq and G12/13 are alpha subunits of the GTP trimer bound protein of animal cells. It does not activate the G11 dependent pathway although G11 is similar to Gq. This toxin binds to an unidentified receptor and then is introduced into the cells by endocytosis and starts to function then. Therefore it does not function on cells without the receptor or defective in the endocytosis pathway.
  • 细胞定位Cytoplasmic

Recombinant Pasteurella multocida toxin protein 图像

  • ab63832 on SDS-PAGE (CBB staining). Molecular Weight: ~145 kDa.
  • Assay of Pasteurella multocida toxin.activity: Induction of aggregation of Swiss 3T3 cells by incubation with PM toxin ab63832.
    1. Control Swiss 3T3 cells
    2. Swiss 3T3 cells incubated with PMT toxin at 10 ng/ml for 20 hours at 37?.
    3. Swiss 3T3 cells incubated with PMT toxin at 100 ng/ml for 20 hours at 37?

Recombinant Pasteurella multocida toxin protein (ab63832)参考文献

ab63832 has not yet been referenced specifically in any publications.

Product Wall



The current lot of Pasteurella multocida toxin protein (Active), ab63832 is provided at a concentration of 0.37mg/ml. However this is lot dependent and may change for future lots. You will always receive a constant quantity of protein (50 &m...

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The Pasteurella mulocida toxin ab63832, or PMT( or dermonecrotic toxin (146 kD)) activates a subunit of the Gq and G12/13 protein which then activates the phospholipase C-b1 that controls
several specific do...

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Te contacto de nuevo ya que he comentado este caso con mis compañeros encargados de la producción y control de calidad de esta proteína.

Este producto se conserva en 50% de glicerol, que tiene una actividad estabilizadora...

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Thank you for contacting us.

Unfortunately, the purification method for ab63832 is proprietary information.

I am sorry for not being able to provide any useful information this time. Please do not hesitate to contact us if you need...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"