重组小鼠Urokinase蛋白(ab92641)
Key features and details
- Expression system: Insect cells
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: ELISA, SDS-PAGE, Functional Studies
描述
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产品名称
重组小鼠Urokinase蛋白
参阅全部 Urokinase 蛋白酶 -
生物活性
The uPA activity of this product was tested using urokinase chromogenic substrate and standardized to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. If you need the specific activity value please contact us with the lot# you require the data for.
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纯度
> 98 % SDS-PAGE. -
表达系统
Insect cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF -
预测分子量
44 kDa -
氨基酸
21 to 433 -
额外的序列信息
(Gene ID: 18792) Full length mature protein, without the signal peptide.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab92641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
ELISA
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
Spectrophotometric Data:
Ultraviolet Absorbance (280nm) = 0.9
epsilon 0.1% = 1.48
ab92641 demonstrates preferential binding to mouse uPAR over Human uPAR coated on an ELISA plate.
Produced using non-baculovirus insect cells.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 5.00
Constituents: 0.41% Sodium acetate, 0.0292% EDTA, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- ATF
- ATF uPA
- BDPLT5
see all -
功能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
组织特异性
Expressed in the prostate gland and prostate cancers. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
细胞定位
Secreted. - Information by UniProt
图片
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SDS-PAGE analysis of mouse urokinase protein (ab92641) under reducing and non-reducing conditions in 12% SDS-PAGE. The urokinase protein is demonstrated to be active through the observed binding to Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein (ab92969) (CPAI protein). CPAI is an inhibitor of urokinase, mutated to be more stable, and the two form a complex that migrates in the gel to a higher molecular weight.
The observed band at 44 kDa in lane 1 corresponds to mouse Urokinase protein under non-reducing conditions.
Lane 1: 3 µg mouse urokinase protein, non-reduced.
Lane 2: + CPAI, 10 µg, non-reduced.Lane 3: Molecular Weight Markers
Lane 4: 3 µg mouse urokinase protein, reduced.
Lane 5: + CPAI, 10 µg, reduced. -
SDS-PAGE analysis of mouse urokinase protein (ab92641) under reducing and non-reducing conditions in 12% SDS-PAGE. The urokinase protein is demonstrated to be active through the observed binding to Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein (ab92969) (CPAI protein). CPAI is an inhibitor of urokinase, mutated to be more stable, and the two form a complex that migrates in the gel to a high molecular weight.
The observed band at 44 kDa in lane 3 corresponds to mouse Urokinase protein under non-reducing conditions.
Lane 1: 5 µg mouse urokinase protein, reduced.
Lane 2: + CPAI, 20 ug, reduced.
Lane 3: 5 ug mouse urokinase protein, non-reduced.
Lane 4: + CPAI, 20 ug, non-reduced.
Lane 5: Prestained Standard Ladder
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab92641 被引用在 1 文献中.
- Semina EV et al. Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma. J Cell Physiol 235:6268-6286 (2020). PubMed: 31990070