重组小鼠TNFSF18/GITRL蛋白(Fc Chimera Active) (ab219721)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组小鼠TNFSF18/GITRL蛋白(Fc Chimera Active)
参阅全部 TNFSF18/GITRL 蛋白酶 -
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Mouse GITR, His Tag at 10μg/mL (100 μL/well) can bind ab219721 with a linear range of 0.007-0.256 μg/mL.
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纯度
> 95 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
TAIESCMVKFELSS SKWHMTSPKP HCVNTTSDGK LKILQSGTYL IYGQVIPVDK KYIKDNAPFV VQIYKKNDVL QTLMNDFQIL PIGGVYELHA GDNIYLKFNS KDHIQKTNTY WGIILMPDLP FIS -
预测分子量
41 kDa including tags -
氨基酸
47 to 173 -
额外的序列信息
This protein carries a human IgG1 Fc tag at the N terminus. (Accession # AAO89011).
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技术指标
Our Abpromise guarantee covers the use of ab219721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride
Lyophilized from 0.22 µm filtered solution.
Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
常规信息
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别名
- Activation inducible TNF related ligand
- Activation-inducible TNF-related ligand
- AITR ligand
see all -
功能
Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues. -
组织特异性
Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. -
序列相似性
Belongs to the tumor necrosis factor family. -
细胞定位
Membrane. - Information by UniProt
图片
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Immobilized Mouse GITR (His Tag) at 5 µg/mL (100 µL/well) can bind Mouse GITR Ligand (Fc Tag) with a linear range of 5-156 ng/mL (QC tested).
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SDS-PAGE analysis of ab219721 under reducing (lane 1) and non-reducing conditions (lane 2); stained overnight with Coomassie Blue.
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Immobilized Mouse GITR, His Tag at 10 μg/mL (100 μL/well) can bind ab219721 with a linear range of 0.007-0.256 μg/mL.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab219721 尚未被引用在任何文献中。