The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The specific activity as determined by its ability to chemoattract human peripheral blood monocytes at 50-100ng/ml corresponding to a Specific Activity of 10,000-20,000IU/mg.
% SDS-PAGE. This protein was purified by proprietary chromatographic techniques.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 10mM Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute with sterile 18MOhm/cm water to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
C-X-C motif chemokine 12
Chemokine (C-X-C motif) ligand 12
Intercrine reduced in hepatomas
Pre-B cell growth-stimulating factor
SDF 1 alpha
Stromal cell derived factor 1
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase.
Belongs to the intercrine alpha (chemokine CxC) family.
Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.