概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP40224
    • 种属Mouse
    • 序列KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
    • 氨基酸22 to 89
    • 额外的序列信息Mature full length for alpha isoform.

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技术指标

Our Abpromise guarantee covers the use of ab51939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50-100 ng/ml.

  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    >99% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng per µg (1EU/µg).
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10mM Acetic acid
    Endotoxin level is <0.1 ng per µg (1EU/µg).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶We recommend a quick spin followed by reconstitution in sterile distilled water to a concentration of 0.1-1.0 mg/ml. (The protein may appear as a haze or film, which is difficult to see at the bottom of the vial.)

常规信息

  • 别名
    • C-X-C motif chemokine 12
    • Chemokine (C-X-C motif) ligand 12
    • cxcl12
    • hIRH
    • hSDF-1
    • Intercrine reduced in hepatomas
    • IRH
    • PBSF
    • Pre-B cell growth-stimulating factor
    • SCYB12
    • SDF 1 alpha
    • SDF-1
    • SDF-1-alpha(3-67)
    • SDF1
    • SDF1_HUMAN
    • SDF1a
    • SDF1B
    • Stromal cell derived factor 1
    • TLSF
    • TPAR1
    see all
  • 功能Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant mouse SDF1 alpha protein (ab51939)参考文献

This product has been referenced in:
  • Wu M  et al. JAM-A promotes wound healing by enhancing both homing and secretory activities of mesenchymal stem cells. Clin Sci (Lond) 129:575-88 (2015). Read more (PubMed: 25994236) »

See 1 Publication for this product

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