Recombinant小鼠SCF protein (ab106309)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Mouse
    • 分子量
      18 kDa
    • 氨基酸
      26 to 190
    • 额外的序列信息
      Murine SCF is a 18.3 kDa protein containing 164 amino acid residues, as the soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

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技术指标

Our Abpromise guarantee covers the use of ab106309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line is < 10 ng/ml, corresponding to a specific activity of >1 x 105 units/mg.
  • 应用

    SDS-PAGE

    Functional Studies

  • 内毒素水平
    < 0.100 Eu/µg
  • 纯度
    > 98 % SDS-PAGE.

  • 形式
    Lyophilised
  • 补充说明

    Endotoxin level: < 0.1 ng/g of SCF

     

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 30mM Tris, 100mM Sodium chloride, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Reconstitute in 50 mM acetic acid or water to a concentration of 0.1 mg/ml. Do not vortex

常规信息

  • 别名
    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • 功能
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 疾病相关
    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • 序列相似性
    Belongs to the SCF family.
  • 发展阶段
    Acts in the early stages of hematopoiesis.
  • 翻译后修饰
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • 细胞定位
    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt

图片

  • 15% SDS-PAGE.
    Lane 1: Molecular weight marker.
    Lane 2: SCF protein.
    Visualised by silver stain.

文献

ab106309 has not yet been referenced specifically in any publications.

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