Recombinant小鼠S100A9 protein (ab109951)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP31725
    • 种属Mouse
    • 序列MGSSHHHHHHSSGLVPRGSHMANKAPSQMERSITTIIDTFHQYSRKEGHP DTLSKKEFRQMVEAQLATFMKKEKRNEALINDIMEDLDTNQDNQLSFEEC MMLMAKLIFACHEKLHENNPRGHGHSHGKGCGK
    • 分子量15 kDa including tags
    • 氨基酸1 to 113
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab109951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab109951 was purified using conventional chromatography.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • Leukocyte L1 complex heavy chain
    • 60B8AG
    • CAGB
    • Calgranulin B
    • Calgranulin-B
    • Calprotectin L1H subunit
    • CFAG
    • CGLB
    • Cystic fibrosis antigen B
    • L1AG
    • Leukocyte L1 complex heavy chain
    • LIAG
    • MAC387
    • MIF
    • Migration inhibitory factor related protein 14
    • Migration inhibitory factor-related protein 14
    • MRP 14
    • MRP-14
    • MRP14
    • Myeloid-related protein 14
    • NIF
    • OTTHUMP00000015331
    • p14
    • Protein S100-A9
    • S100 A9
    • S100 calcium binding protein A9
    • S100 calcium binding protein A9 calgranulin B
    • S100 calcium-binding protein A9
    • S100A9
    • S10A9_HUMAN
    see all
  • 功能Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • 组织特异性Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • 序列相似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 翻译后修饰Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • 细胞定位Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt

Recombinant Mouse S100A9 protein 图像

  • 15% SDS-PAGE analysis of ab109951 (3µg)

Recombinant Mouse S100A9 protein (ab109951)参考文献

ab109951 has not yet been referenced specifically in any publications.

Product Wall

The laboratory tested the endotoxin level of ab109951, and it’s below (<) 1EU/ug.

We are sorry that we cannot be of more help on this occasion. We - the technical team - would like to wish you good luck with your research, and are always at your service, should you require further expert advice. Have a great weekend!

Thank you for your inquiry. My colleague is currently on tour for Abcam in Germany, but before she left she inquired about the endotoxin level of S100A9 protein ab109951. I was informed that, regrettably, the Endotoxin concentration of this prote...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"