Recombinant小鼠PKM2 protein (ab95474)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Mouse

技术指标

Our Abpromise guarantee covers the use of ab95474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: 11 pmol/min/µg. A 50 µl PKM2 reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 100mM MgCl2, 250 mM KCl, 40 mM ADP, 100 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected using Kinase-Glo Luminescent Kinase Assay Platform.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CTHBP
    • Cytosolic thyroid hormone binding protein
    • Cytosolic thyroid hormone-binding protein
    • KPYM_HUMAN
    • MGC3932
    • OIP 3
    • OIP-3
    • OIP3
    • OPA interacting protein 3
    • Opa-interacting protein 3
    • p58
    • PK muscle type
    • PK, muscle type
    • PK2
    • PK3
    • PKM
    • PKM2
    • pykm
    • Pyruvate kinase 2/3
    • Pyruvate kinase 3
    • Pyruvate kinase isozymes M1/M2
    • Pyruvate kinase muscle
    • Pyruvate kinase muscle isozyme
    • pyruvate kinase PKM
    • Pyruvate kinase, muscle 2
    • TCB
    • THBP1
    • Thyroid hormone binding protein 1
    • Thyroid hormone binding protein cytosolic
    • Thyroid hormone-binding protein 1
    • Tumor M2 PK
    • Tumor M2-PK
    see all
  • 功能Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • 组织特异性Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 序列相似性Belongs to the pyruvate kinase family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 细胞定位Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt

Recombinant mouse PKM2 protein 图像

  • Specific activity curve of ab95474
  • 10% SDS-PAGE
    Lane 1: 10µg ab95474
    Lane 2: Molecular Weight markers

Recombinant mouse PKM2 protein (ab95474)参考文献

ab95474 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95474.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"