- 数据表
- 具体的参考文献 (3)
- 实验方案
概述
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产品名称重组小鼠MMP9蛋白
参阅全部 MMP9 蛋白酶 -
蛋白长度Full length protein
描述
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性质Recombinant
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来源BHK cells
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氨基酸序列
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种属Mouse
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab39309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式Liquid
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补充说明
Mouse-MMP9 degrades denatured collagen (gelatin), and a range of extracellular matrix components in-vivo. Unlike MMP2, MMP9 is not constituitively produced by most normal cells, although the enzyme is often over expressed by transformed cells, tumor cells, and cells treated with the phorbol ester PMA. An endogenous inhibitor to MMP9, TIMP1, is often complexed with the enzyme in-vivo, but has been removed from this preparation.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 50% Glycerol, PBS, 250mM Sodium chloride, 0.6 % DMSO, pH 7.4
常规信息
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别名
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
see all -
功能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
组织特异性Produced by normal alveolar macrophages and granulocytes.
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疾病相关Intervertebral disc disease
Metaphyseal anadysplasia 2 -
序列相似性Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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翻译后修饰Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
细胞定位Secreted, extracellular space, extracellular matrix.
- Information by UniProt
图片
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Western blot - Recombinant Mouse MMP9 protein (ab39309)All lanes : Anti-MMP9 antibody (ab38898) at 2 µg/ml
Lane 1 :Natural human MMP9 protein (Proenzyme, monomer) (ab157344)
Lane 2 :Recombinant Mouse MMP9 protein (ab39309)
Lysates/proteins at 0.1 µg per lane.
Performed under reducing conditions.This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with anti-MMP9 antibody (ab38898; 2 microgram per mL) overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody (diluted 1:20000) for 1 hour at room temperature before imaging.
文献
This product has been referenced in:
- Chiang HY et al. R1R2 peptide ameliorates pulmonary fibrosis in mice through fibrocyte migration and differentiation. PLoS One 12:e0185811 (2017). Read more (PubMed: 28968441) »
- Nighot P et al. Matrix metalloproteinase 9-induced increase in intestinal epithelial tight junction permeability contributes to the severity of experimental DSS colitis. Am J Physiol Gastrointest Liver Physiol 309:G988-97 (2015). Read more (PubMed: 26514773) »
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