Recombinant小鼠Interferon gamma protein (ab123747)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Mouse
    • 分子量
      16 kDa
    • 氨基酸
      23 to 155

技术指标

Our Abpromise guarantee covers the use of ab123747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Biological Activity: The ED50 as determined by a cytopathic affect inhibition assay with murine NIH 3T3 cells challenged with VSV virus is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
  • 应用

    Functional Studies

    SDS-PAGE

    HPLC

  • 纯度
    > 95 % SDS-PAGE.
    Purity by SDS-PAGE and HPLC: 95% Endotoxin level is <0.1 ng per µg of IFN gamma.
  • 形式
    Lyophilised
  • 补充说明
    Endotoxin Level: <0.1 ng/µg.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20ºC.

    pH: 7.00
    Constituents: 0.16% Sodium phosphate, 0.15% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Reconstitute in ddH2O to a concentration of 1 mg/ml. Further dilutions should be made into RPMI + 10% FCS and stored at 4°C for 1 week or –20°C for future use.

常规信息

  • 别名
    • IFG
    • IFI
    • IFN gamma
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    see all
  • 功能
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 组织特异性
    Released primarily from activated T lymphocytes.
  • 疾病相关
    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • 序列相似性
    Belongs to the type II (or gamma) interferon family.
  • 翻译后修饰
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • 细胞定位
    Secreted.
  • Information by UniProt

文献

ab123747 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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