The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The activity is determined by the dose dependent production of IL-8 from human PBMCs and is typically less than 25 ng/mL.
< 0.100 Eu/µg
% SDS-PAGE. Protein content and purity of ab119155 was determined by UV spectroscopy at 280 nm, RP-HPLC calibrated against a known standard and quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
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Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
pH: 7.50 Constituent: 0.14% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Lyophilized product is very stable at -20°C. Centrifuge vial before opening. When reconstituting, gently pipette and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute ab119155 with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
ab119155 used in Western Blot. Figure: 1 ug run under (+) reducing conditions and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse IL-31 has a predicted MW of 15.7 kDa.
Recombinant mouse IL31 protein (ab119155)参考文献
has not yet been referenced specifically in any publications.