重组小鼠IL-31蛋白(ab119155)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC
描述
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产品名称
重组小鼠IL-31蛋白
参阅全部 IL-31 蛋白酶 -
生物活性
The activity is determined by the dose dependent production of IL-8 from human PBMCs and is typically less than 25 ng/mL. -
纯度
> 95 % SDS-PAGE.
Protein content and purity of ab119155 was determined by UV spectroscopy at 280 nm, RP-HPLC calibrated against a known standard and quantitation against a known standard via reducing and non-reducing SDS-PAGE gels. Purified using standard chromatographical techniques. -
内毒素水平
< 0.100 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
MTCSLSFGAPISKEDLRTTIDLLKQESQDLYNNYSIKQASGMSADESIQL PCFSLDREALTNISVIIAHLEKVKVLSENTVDTSWVIRWLTNISCFNPLN LNISVPGNTDESYDCKVFVLTVLKQFSNCMAELQAKDNTTC -
预测分子量
16 kDa -
氨基酸
24 to 163
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技术指标
Our Abpromise guarantee covers the use of ab119155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
HPLC
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形式
Lyophilized -
补充说明
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituent: 0.14% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Lyophilized product is very stable at -20°C. Centrifuge vial before opening. When reconstituting, gently pipette and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute ab119155 with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
常规信息
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别名
- IL 31
- IL-31
- IL31
see all -
功能
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. -
组织特异性
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. -
细胞定位
Secreted. - Information by UniProt
图片
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SDS PAGE analysis of ab119155 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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ab119155 used in Western Blot. Figure: 1 ug run under (+) reducing conditions and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse IL-31 has a predicted MW of 15.7 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab119155 尚未被引用在任何文献中。