The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Purity by SDS-PAGE and HPLC: = or > 95%
Endotoxin level is < 0.01 ng per µg of ab119730.
The lyophilized ab119730 is best-stored desiccated at -20 °C. Upon reconstitution the product should be aliquoted and stored at -20 °C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
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Shipped at 4°C. Store at -20°C.
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 5 mM Acetic acid to a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.